Plasma for pups (Cure for fading puppy syndrome) No Colostrum no problem!

Giving puppies whole plasma, 3cc’s per pound of puppy weight when they have not gotten colostrum (kept in the fridge it will stay good for years)—The dose is 5 cc per puppy 3 times over a 24 hour period. If this can be administered in the first 24 hours after birth, it can be given orally with a feeding tube. After the pups are 24 hours old, it must be given by subq or IO injection to be effective.

It has been found that frozen plasma is the only natural immune booster for newborn pups. Breeders have reported that pups fed plasma in the first 48 hours show consistent weight gain and are more vigorous than previous litters.

"Fading pups" are never a concern with pups boosted with plasma. Because the newborn's digestive system is not completely operational for the first hours after birth, the molecular immune boosting components of the frozen plasma pass unobstructed into the pup's circulation.

If the bitch has a caesarian section, providing frozen plasma for the pups becomes even more important as the quality of the bitch's first colostrum may by compromised due to post-surgical antibiotic therapy.

IF YOU ARE DRAWING YOUR OWN BLOOD REMBER DO NOT DRAW MORE THAN 5ML/CC PER POUND OF BODY WEIGHT. Then give a 14 day rest.

As per Indiana university

https://research.iu.edu/doc/compliance/animal-care/bloomington/iub-biacuc-blood-collection-in-laboratory-animals.pdf

HELPFUL HINTS FOR USING THE FROZEN PLASMA FOR NEWBORN PUPS
a. Keep plasma frozen until bitch starts to whelp. b. Warm plasma to body temperature before administering. c. Give to each pup orally, several drops at a time, every two hours up to three cc per one pound of body weight over the first 24 hours following whelp. Using a new eye dropper for administrating has been recommended by owners.	d. Dose after pup is warm, dry, and suckling. e. Refrigerate plasma between use. Use any remaining plasma, the second twenty-four hours in the manner described above.

Instructions

Treatment of Newborn Puppies Using
Fresh-Frozen Plasma during the First 10 Days of Life
For this purpose, Fresh-Frozen Plasma comes in 10-12 cc (ml) plastic tubes. If purchased, This product needs to be sent frozen by priority overnight courier.

The recommended dosage of 3 to 5 cc (ml) per pound can be given to each puppy orally (by mouth) or IP (intraperitoneally, by your vet or with his instruction) in the first 24-36 hours.

Beyond 36 hours of life the plasma must be given intraperitoneally or intravenously. (check with your veterinarian)

Usually the pups only need to be treated once. However, if they appear to be fading, the dose can be repeated in 5-7 days later if necessary, but it must be IP or IV.

Do not give more than 10cc(ml) at one time.

Fading Puppy Syndrome
Fresh-Frozen Plasma can also be given at any time during the first 10 days of life if newborns appear to be fading for any reason.
3-4 cc (ml) per puppy for tiny puppies
3-5 cc (ml) per pound for larger puppies

Veterinarians:
The Treatment of Puppies Using Fresh-Frozen Plasma to Counteract Parvo Virus
A. Thaw plasma in warm (not hot) water.
B. Administer intravenously at a dosage of 3 to 5 cc (ml) per pound

TOTAL 5 YEAR SHELF LIFE

TREATING “FADING PUPPY SYNDROME” or ORPHAN PUPS with PLASMA One important use of blood plasma is to provide a source of globulins (plasma protein antibodies) to protect weak, fading or orphan newborns against the common infectious agents to which they are exposed. Plasma treatment [canine fresh-frozen plasma (FFP)] for orphaned puppies or for those receiving only minimal colostrum after birth should be given three times in the first 24 - 48 hrs of life (1st at birth, 2nd in 12 hours and 3rd time in 12 hours). Treatment for healthy newborns may be repeated at 5 to 14 days of age and then again at 3 to 4 weeks of age. For sick newborns, more frequent transfusions of FFP may be necessary. These transfusions are usually given intraperitoneally (IP), but they can also be given orally (by mouth) in the first 24-36 hours of life [as FFP is salty, it should be followed with a little drop of honey or syrup on the tongue]. When puppies are two days of age or older, the route of administration must be IP (or IV or subcutaneously) and not oral as the antibodies in plasma will no longer be absorbed through the gastrointestinal tract. The recommended dose is 3-5 mL per pound of body weight: 0.25 x Weight of Puppy in Ounces = The Amount of Plasma given in mL or cc. This is given to each puppy orally, IP or subcutaneously. Do not give more than 10 mL at one time. Do not mix FFP with any solution, including formula, Lactated Ringers, water, etc. The plasma by itself is very stable, but addition of any foreign solution may adversely affect the chemical composition of the plasma. FFP can be refrozen after thawing without loss of viability. Check the screw top of the tube while thawing as it can loosen and drip contents. After thawing, a tube can be re-frozen as long as it has not been left out at room temperature for more than 1 hour. Similarly, if only part of a tube is used or needed, the remainder of the tube can be placed in the refrigerator for 24 hours and then should be re-frozen. There will not be any loss of albumin and globulin activities for up to 5 years, however, coagulation factors, which are typically used for bleeding disorders not found in newborns, diminish after 1 year. -

References

Dodds, WJ. 1993. Known medical indications for using fresh-frozen plasma. DVM Newsmagazine 24(4): 42-43.
Poffenberger EM, Olson, PN, Chandler, ML, et al. 1991. Use of adult dog serum as a substitute for colostrum in the neonatal dog. Am J Vet Res 52: 1221-1224.

Bouchard, G, Plata-Madrid, H, Youngquist, RS et al. 1992. Absorption of an alternate source of immunoglobulin in pups. Am J Vet Res 53: 230-233.

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